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產(chǎn)品中心您現(xiàn)在的位置:首頁(yè) > 產(chǎn)品展示 > 生物試劑 > Beckman > B53230質(zhì)控校準(zhǔn)微球CytoFLEX Daily QC Fluoroscence beads

B53230質(zhì)控校準(zhǔn)微球CytoFLEX Daily QC Fluoroscence beads

更新時(shí)間:2025-06-06

簡(jiǎn)要描述:

產(chǎn)品簡(jiǎn)介:CytoFLEXDailyQCFluorospheres是熒光微球的懸浮液,可用于日常驗(yàn)證CytoFLEX流式細(xì)胞儀的光學(xué)校準(zhǔn)和流體系統(tǒng)

產(chǎn)品簡(jiǎn)介:

CytoFLEX Daily QC Fluorospheres 是熒光微球的懸浮液,可用于日常驗(yàn)證 CytoFLEX 流式細(xì)胞儀的光學(xué)校準(zhǔn)和流體系統(tǒng)。


試劑組分:

CytoFLEX Daily QC 熒光球由約 3 µm 熒光球的懸浮液組成,當(dāng)在 405 nm、488 nm 或 635 nm 激發(fā)時(shí),其熒光發(fā)射范圍為 410 nm 至 800 nm。 將混合物懸浮在含有表面活性劑和防腐劑的水性介質(zhì)中。


注意事項(xiàng):

1. 本產(chǎn)品只能在 CytoFLEX 鞘液 (PN B51503) 或 RO/DI(反滲透/去離子)水中稀釋。 添加有機(jī)溶劑或高離子強(qiáng)度溶液可能會(huì)不可逆地溶脹或聚集熒光球。

2. 熒光球會(huì)長(zhǎng)時(shí)間沉降。 確保熒光球在使用前重新懸浮。

3. 不要使用超過小瓶標(biāo)簽上的失效日期的熒光球。

4. 處理該試劑時(shí)使用良好實(shí)驗(yàn)室規(guī)范 (GLP)。


儲(chǔ)存條件:

當(dāng)儲(chǔ)存在 2-8°C 時(shí),該試劑在小瓶標(biāo)簽上的有效期內(nèi)是穩(wěn)定的。 不要凍結(jié)。 盡量減少光照。 打開的小瓶和準(zhǔn)備好的熒光球必須在使用后冷藏。


試劑制備:

使用前劇烈混合。 將三 (3) 滴 CytoFLEX Daily QC Fluorospheres 添加到 1.0 mL CytoFLEX 鞘液或 RO/DI 水中并混合。 制備的熒光球在 2-8°C 下儲(chǔ)存時(shí),稀釋后最多可使用五天。


校準(zhǔn)步驟:

1. 使用制造商推薦的濾光片組來檢測(cè)合適的熒光參數(shù)(參考 CytoFLEX 儀器 IFU)。

2. 確保特定批次 CytoFLEX Daily QC Fluorospheres 的目標(biāo)值文件已加載到流式細(xì)胞儀軟件中。 有關(guān)導(dǎo)入批次特定目標(biāo)值文件的說明,請(qǐng)參閱您的 CytoFLEX 儀器 IFU。

3. 用力混合制備好的 CytoFLEX Daily QC Fluorospheres 樣品并放入流式細(xì)胞儀中。

4. 按照 CytoFLEX 儀器 IFU 中的說明運(yùn)行質(zhì)量控制 (QC) 協(xié)議。


英文介紹:

CytoFLEX Daily QC Fluorospheres consists of a suspension of ~3 μm fluorospheres with a fluorescence emission of 410 nm to 800 nm when excited at 405 nm, 488 nm or 635 nm. CytoFLEX Daily QC Fluorospheres may be used for daily verification of the CytoFLEX flow cytometer's optical alignment and fluidics system.

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