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技術(shù)文章您現(xiàn)在的位置:首頁 > 技術(shù)文章 > RNA、DNA和蛋白質(zhì)和細(xì)胞完整性保存液CellCover

RNA、DNA和蛋白質(zhì)和細(xì)胞完整性保存液CellCover

更新時間:2023-01-01   點擊次數(shù):1936次

CellCover是一種即用型溶液,用于同時保護(hù)人類和動物實體組織中的 RNA、DNA 和蛋白質(zhì),包括腫瘤、白細(xì)胞和培養(yǎng)細(xì)胞(貼壁、懸浮液、球狀體)。Ready to use solution for complete protecting of RNA, DNA and protein in human and animal solid tissues, including tumors, white blood cells and cultured cells (adherent, suspension, spheroids).

Anacyte Laboratories多年只專注于一款產(chǎn)品,就是細(xì)胞/組織原生狀態(tài)保存產(chǎn)品CellCover, 是一款即用的,無毒的液體固定液,隨著細(xì)胞與CellCover的接觸,其代謝狀態(tài)被即刻凍結(jié),生物大分子的合成和轉(zhuǎn)運活動立即停止;細(xì)胞內(nèi)各種降解通路即時中斷,化學(xué)降解同樣被迅速抑制而終止;由此保證DNA、RNA和蛋白質(zhì)等生物大分子的穩(wěn)定性。在不需要化學(xué)交聯(lián)的情況下保持細(xì)胞形狀的完整性。用于保護(hù)包括腫瘤、白細(xì)胞和培養(yǎng)細(xì)胞(貼壁、懸浮、球狀體)的RNA、DNA和蛋白質(zhì)和細(xì)胞形狀的完整性。


CellCover 是允許在細(xì)胞生理/病理狀態(tài)環(huán)境中即刻并行存儲蛋白質(zhì)、RNA 和 DNA 的試劑。

* 保持細(xì)胞形狀完整性,無需化學(xué)交聯(lián):在幾乎任何基質(zhì)上生長的細(xì)胞都可以用 CellCover 通過液體冷凍進(jìn)行處理,以保持體內(nèi)形態(tài),而不會 在細(xì)胞固定過程中發(fā)生生物分子的化學(xué)交聯(lián)。

* 保護(hù) DNA,RNA 和蛋白質(zhì)、蛋白質(zhì)表達(dá)和基因表達(dá)狀態(tài):旨在保護(hù)人類和動物細(xì)胞和實體組織(包括腫瘤和培養(yǎng)細(xì)胞(貼壁細(xì)胞、懸浮細(xì)胞、球體))中 DNA、RNA 和蛋白質(zhì)表達(dá)和基因表達(dá)的狀態(tài)。

*一步快速穩(wěn)定: CellCover 是為  生命科學(xué)研究中生物分子的快速“一步"穩(wěn)定而開發(fā)的,使用 CellCover 進(jìn)行 RNA 固定并充當(dāng) RNA 穩(wěn)定劑,可抑制 RNA 降解,從而在 RNA 測序或單細(xì)胞 RNA seq 或單細(xì)胞測序?qū)嶒炛腥〉煤芎玫慕Y(jié)果(高RIN值)。

* 無毒,即用型溶液: CellCover 是一種無毒配方,是甲醛的替代品,用于人類和動物細(xì)胞和實體組織,包括腫瘤和培養(yǎng)細(xì)胞(貼壁細(xì)胞、懸浮細(xì)胞、球體)

* 用于基因組分析、轉(zhuǎn)錄組分析和蛋白質(zhì)組分析的真正具有代表性的分子:CellCover 充當(dāng) DNA 和 RNA 穩(wěn)定劑和屏蔽劑,例如 用于通過流式細(xì)胞術(shù)或 NGS 或 RNA seq 方法進(jìn)行分析。CellCover 是基因組分析、轉(zhuǎn)錄組分析和蛋白質(zhì)組分析成功實驗的關(guān)鍵初始步驟。

* 幾乎所有下游應(yīng)用都與使用 CellCover 的細(xì)胞固定兼容,包括單細(xì)胞 RNA 測序、NGS、蛋白質(zhì)測序等:


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靶點科技(北京)有限公司

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